Toxicological evaluation of a wastewater treatment intensifier and livestock waste biodegrader

Abstract

This paper presents the results of new studies of microbiologically synthesized products containing  microbial  cells  of the genus Bacillus as an active ingredient (hereinafter referred to as a. i.) – an intensifier of wastewater treatment processes and a biodegrader of manure. The clinical picture of acute poisoning was studied, and acute toxicity parameters were established following a single intragastric administration and cutaneous application to white rats. The sensitizing effect on white mice, the irritant effect following a single application to intact skin of white rats, and the irritant effect following a single application to the mucous membranes of the eyes of albino rabbits were studied. The characteristics of the toxic effect following repeated intragastric administration to white rats were also examined. Based on the results obtained under acute and subacute experimental conditions, it was established that the manure biodestructor (a. i., microbial cells of the genus Bacillus at a concentration of ≥ 10⁷ colony-forming unit per gram (hereinafter referred to as CFU/g) and bioactivator (a. i., microbial cells of the genus Bacillus at a concentration of ≥ 10⁹ CFU/g) do not have a general toxic or specific effect on the body of warm-blooded animals. Experimental data indicate a favorable safety profile of the studied biologics and allow them to be classified as products with an acceptable level of risk to human health when used for their intended purpose in the agro-industrial complex, in compliance with established hygienic regulations and application recommendations.