Ignatenko-en | Chemical Safety Science

Chemical Safety Science, 2018, Volume 2, No 2, p. 251 — 272

Utilization and biodegradation of wastes

UDC 628.38:615.9 Download PDF (RUS)

DOI: 10.25514/CHS.2018.2.14120

SAMPLE PREPARATION AND BIOTESTING TOXICITY OF SEWAGE SLUDGE WASTES

I. V. Ignatenko

Belarusian State Technological University, Department of Biotechnology, Minsk, Republic of Belarus

Received November 23, 2018

Published December 26, 2018

Abstract – Potential benefits and drawbacks of physicochemical and biological approaches for evaluating toxicity of sewage sludge are considered. A procedure for sample preparation and biotesting of sewage sludge toxicity by survival test with Euglena gracilis cells has been developed. The toxicity analysis of hydrophobic and hydrophilic substances isolated from sediments by hexane and bile extraction has been carried out. A sequential scheme of water extraction followed by stepwise heat treatment of sewage sludge at 20°C, 100°С, 270°С, and 550°С is proposed for analysis of free and bound forms of heavy metals. The proposed approach for assessment of current and potential chemical hazard of sewage sludge wastes will allow to estimate their safety and select an appropriate procedure for their detoxification and subsequent treatment.

Keywords: sewage sludge, chemical safety, toxicity, sample preparation, toxic substances, heavy metals, biotesting, survival test, Euglena gracilis cells.

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